Relationship of folate metabolism related enzymes MTHFR and MTRR gene polymorphisms with unexplained recurrent spontaneous abortion

The role of methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C, methioninesynthase reductase (MTRR) A66G was unclear in repeated spontaneous abortion. To provide experimental basis for etiological diagnosis and treatment of recurrent spontaneous abortion, we selected 197 patients with recurrent spontaneous abortion and 116 normal women, extracting the oral mucosal epithelial cells and detect to the MTHFR gene C677T, A1298C and MTRR gene loci of A66G single nucleotide polymorphisms. The result showed that compared with the control group, the distribution of C677T MTHFR allele in URSA group was statistically significant (P < 0.05). The frequency of TT allele was higher than that of the control group, and the frequency of CT allele was lower than that of the control group. MTHFR A1298C and MTRR mutation frequency between the URSA group and the control group no significant difference (P > 0.05); URSA group the activity of MTHFR, red cell folate and plasma folate levels lower than control group (P < 0.05) and homocysteine levels higher than control group (P < 0.05); URSA patients in different ages of human serum folate and red cell folate, homocysteine homocysteine levels does not exist significant difference (P > 0.05). We considered that the mutation of C677T gene in MTHFR gene is associated with the risk of URSA, which suggests that TT allele may be a susceptibility gene of URSA. URSA group in age and plasma folate and red cell folate and homocysteine homocysteine levels is not relevant, which for pregnant and maternal folic acid supplement guidance and monitoring, so as to further reduce recurrent spontaneous abortion rate has important significance.